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Loss of epithelial barrier integrity is a hallmark of airway injury and occurs in airway diseases including COPD, acute respiratory distress syndrome, asthma, fibrosis, and EMT/cancer, thus supporting the conclusion that the application of liquid to dpHBEC causes a phenotype that is consistent with a range of respiratory diseases (Olson et al., 2011)

However, the reason for the emergence of OSCC in NSNDs is still largely unknown

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