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It took more than 7,000 scientific studies and the death of countless smokers before the first Surgeon Generals report against smoking was released in the 1960s

Absorbance of each well was quantified with a plate reader (Victor 2, PerkinElmer Life Science/Wallac Oy, Turku, Finland) at 550 and 650 nm, and the background at 650 nm was subtracted from the absorbance at 550 nm

Then try to think of alternative behaviours to replace reaching for a cigarette

Gas Chromatography I Mass Spectrometry (GC-MS), Liquid Chromatography I Mass Spectrometry (LC-MS), and their tandem mass-spectrometer versions are the preferred confirmatory methods