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Method The intermediate components believed to originate from tobacco alkaloids in mainstream cigarette smoke were determined using as gas-chromatography hyphenated to a mass spectrometer fitted with a mass selective detector (MSD) while the energetics of intermediates were conducted using the density functional theory framework (DFT/B3LYP) using the 6-31G basis set

While high dosages of nicotine support atherogenic improvements in several models, most existing research shows that nicotine has a slight impact on the onset or spread of atherosclerosis at concentrations comparable to those of a smokers blood level (31, 57)

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