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US regulators probe reports of e-cigarette use causing seizures US regulators said Wednesday they are investigating reports that e-cigarettes -- increasingly widespread in America and elsewhere -- may cause seizures."We have reports indicating that some people who use e-cigarettes, especially youth and young adults, are experiencing seizures following their use," the Food and Drug Administration (FDA) said in a statement.The FDA has reviewed "voluntary adverse event reports," finding "a total of 35 reported cases of seizures following use of e-cigarettes between 2010 and early 2019," it said."We believe these 35 cases warrant scientific investigation into whether there is in fact a connection." The use of e-cigarettes -- colloquially known as vaping -- began to take off among young Americans in the 2010s, and overtook cigarette smoking in 2014.The US categorizes e-cigarettes -- which deliver nicotine, often as part of fruit or sweets-flavored vapor -- as tobacco products, a definition not shared by all countries.The use of e-cigarettes -- colloquially known as vaping -- began to take off among young Americans in the 2010s, and overtook cigarette smoking in 2014Charts showing the prevalence of e-cigarettes and conventional cigarettes in the US high schools

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Immediately after, the plasma was separated via centrifugation (1.5 rpm for 15 min at 4C)