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Gastric acid or bile travels backward into the esophagus, producing an unpleasant sour or bitter taste
In vitro transcription Capped mRNA was transcribed in vitro in the presence of the cap analogs m 7 G[5]ppp[5]G or m 2 7,3-O G[5]ppp[5]G (New England Biolabs, Ipswich, MA, USA) using the T7 High Yield RNA Synthesis Kit (New England Biolabs) and DNA templates amplified by PCR from the constructs described above using Phusion Polymerase (New England Biolabs) and the following primers (Additional file 2): 10 and 13 for pIVEX1.3_eYFP-His, pIVEX1.3_His-eYFP, pCITE2a_His-eYFP, pIVEX1.3_FFLuc-His and pIVEX1.3_RRLuc-His
When the camp followers reached Ivrea, Beyle immediately went looking for the towns opera house
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