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Design Details I had been driving by this old barn south of McFarland with the Chew Tobacco advertisement painted on its north face (Tobacco is almost

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Membranes were blocked with 5% BSA in Tris-buffered saline with 0.1% Tween-20 (TBS-T) and incubated at 4 C overnight with the following antibodies: anti-GOT2 (Proteintech, 14800-1-AP, 1:1,000 dilution), anti-GOT1 (Cell Signalling, 34423S, 1:1,000 dilution), anti-GFP (Sigma, 11814460001, 1:1,000 dilution), anti-FH (Origene, TA500675S, 1:1,000 dilution), anti-SDHB (Atlas, HPA002868, 1:1,000 dilution), anti-pChk1 (Cell Signalling, 2348S, 1:1,000 dilution), anti-Chk1 (Cell Signalling, 2G1D5, 1:1,000 dilution), anti-pChk2 (Cell Signalling, 2197S, 1:1,000 dilution), anti-GAPDH (Cell Signalling, 5174S, 1:5,000 dilution), anti-Vinculin (Sigma, SAB4200729, 1:10,000 dilution) and anti-tubulin (Sigma, T6199)